验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue, pig skeletal muscle tissue, mouse skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67299-1-Ig targets MYH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, chicken |
| 免疫原 |
CatNo: Ag17129 Product name: Recombinant human MYH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC114545 Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | myosin, heavy chain 1, skeletal muscle, adult |
| 别名 | 1G10H9, MyHC-2x, MyHC-IIx/d, Myosin 1, Myosin-1 |
| 计算分子量 | 1939 aa, 223 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | BC114545 |
| 基因名称 | MYH1 |
| Gene ID (NCBI) | 4619 |
| RRID | AB_2882563 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P12882 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| IHC protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| WB protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Cancer IDO1-Mediated Tryptophan-Kynurenine Metabolic Reprogramming to Drive Skeletal Muscle Atrophy and Cachexia Acceleration. | ||
Int J Biol Macromol LncRNA lncMGR regulates skeletal muscle development and regeneration by recruiting CDK9 and sponging miRNAs | ||
Food Funct Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model. | ||
Cells Ambient Temperature Shapes Skeletal Muscle Growth and Fiber-Type Plasticity in Mice. | ||
Nutrients Sodium Butyrate Alleviates Heat Stress-Induced Oxidative Stress and Skeletal Muscle Homeostasis Disruption by Promoting Autophagy in Mice | ||
Int J Mol Sci Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway |
