DDX4,VASA Monoclonal antibody

DDX4,VASA Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67147-2-Ig

产品说明书

CloneNo. 1F12C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, IHC, IF-P, ELISA and More (1)

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSKOV-3 cells, JAR cells, rat testis tissue, NCCIT cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 3 publications below

产品信息

67147-2-Ig targets DDX4,VASA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 DDX4,VASA fusion protein Ag11630 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
别名 DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog
计算分子量690aa,76 kDa; 724aa,79 kDa
观测分子量79 kDa
GenBank蛋白编号BC047455
基因名称 DDX4
Gene ID (NCBI) 54514
RRIDAB_2882446
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

实验方案

Product Specific Protocols
WB protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
IHC protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
IF protocol for DDX4,VASA antibody 67147-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Reproduction

NR2C2 of macrophages promotes inflammation via NF-κB in LPS-induced orchitis in mice

Authors - Ziqian Min
ratIF

Reprod Sci

The Mechanistic Study of Mitochondrial Autophagy and Ferroptosis in the Progression of Decreased Ovarian Reserve

Authors - Qianwen Ma
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
ratIF

Reprod Sci

Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy

Authors - Qianwen Ma
Loading...