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验证数据展示

  • WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

    WB analysis of SKOV-3 using 67147-2-Ig (same clone as 67147-2-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    IHC staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    IF Staining of mouse testis using 67147-2-Ig (same clone as 67147-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (67147-2-Ig, Clone: 1F12C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67147-2-PBS in a different storage buffer formulation.

DDX4,VASA Monoclonal antibody, PBS Only

DDX4,VASA Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67147-2-PBS

产品说明书

CloneNo. 1F12C3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67147-2-PBS targets DDX4,VASA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 DDX4,VASA fusion protein Ag11630 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
别名 DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog
计算分子量690aa,76 kDa; 724aa,79 kDa
观测分子量79 kDa
GenBank蛋白编号BC047455
基因名称 DDX4
Gene ID (NCBI) 54514
RRIDAB_2882446
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQI0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].