CoraLite® Plus 488-conjugated DDX4,VASA Monoclonal antibody
DDX4,VASA Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F12C3
验证数据展示
经过测试的应用
| Positive IF detected in | mouse testis tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67147 targets DDX4,VASA in IF applications and shows reactivity with Human, mouse samples.
| Tested Applications | IF-P |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | mouse |
| Immunogen | DDX4,VASA fusion protein Ag11630 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| Synonyms | DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog |
| Calculated Molecular Weight | 690aa,76 kDa; 724aa,79 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC047455 |
| Gene Symbol | DDX4 |
| Gene ID (NCBI) | 54514 |
| RRID | AB_2919428 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NQI0 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DDX4,VASA antibody CL488-67147 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Sevoflurane inhibited reproductive function in male mice by reducing oxidative phosphorylation through inducing iron deficiency |