验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67147 targets DDX4,VASA in IF-P applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF-P Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | DDX4,VASA fusion protein Ag11630 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| 别名 | DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog |
| 计算分子量 | 690aa,76 kDa; 724aa,79 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC047455 |
| 基因名称 | DDX4 |
| Gene ID (NCBI) | 54514 |
| RRID | AB_2919428 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NQI0 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DDX4,VASA antibody CL488-67147 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |