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验证数据展示

  • WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    WB analysis using 66989-1-Ig (same clone as 66989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IST1 antibody included
  • IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    IHC staining of human lung cancer using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IST1 antibody included
  • IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    IF Staining of HeLa using 66989-1-Ig (same clone as 66989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66989-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51578-1

    Cytometric bead array sample test of MP51578-1

    Cytometric bead array sample test of MP51578-1, hIST1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66989-1-PBS. Detection antibody: 66989-2-PBS.

  • Cytometric bead array standard curve of MP51578-1

    Cytometric bead array standard curve of MP51578-1

    Cytometric bead array standard curve of MP51578-1, hIST1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66989-1-PBS. Detection antibody: 66989-2-PBS. Standard:Ag28364. Range: 3.125-400 ng/mL.

hIST1 Monoclonal antibody, PBS Only (Capture)

hIST1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66989-1-PBS

产品说明书

CloneNo. 1E12A7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

IST1, KIAA0174, 1E12A7, Charged multivesicular body protein 8, CHMP8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IST1 Ready-To-Use IHC Kit. IST1 primary antibody included.

产品信息

66989-1-PBS targets hIST1 as part of a matched antibody pair:

MP51578-1: 66989-1-PBS capture and 66989-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28364

Product name: Recombinant human hIST1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-360 aa of BC000430

Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0174
别名 IST1, KIAA0174, 1E12A7, Charged multivesicular body protein 8, CHMP8
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC000430
基因名称 hIST1
Gene ID (NCBI) 9798
RRIDAB_2882306
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53990
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

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