验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, mouse liver tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, A549 cells, HepG2 cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66989-1-Ig targets hIST1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag28364 Product name: Recombinant human hIST1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-360 aa of BC000430 Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0174 |
| 别名 | IST1, KIAA0174, 1E12A7, Charged multivesicular body protein 8, CHMP8 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC000430 |
| 基因名称 | hIST1 |
| Gene ID (NCBI) | 9798 |
| RRID | AB_2882306 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P53990 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| IHC protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| WB protocol for hIST1 antibody 66989-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains | ||
J Cell Biol Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters | ||
Adv Biol (Weinh) What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation? | ||
Mol Biol Cell Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission. | ||
bioRxiv VPS4 and CHMP7 release centromeres from the nuclear envelope for post-mitotic positioning in daughter nuclei. |
