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hIST1 Monoclonal antibody

hIST1 Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1E12A7

Cat No : 66989-1-Ig

Print datasheet

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

验证数据展示

  • WB analysis using 66989-1-Ig

    WB analysis using 66989-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66989-1-Ig (hIST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66989-1-Ig

    IHC staining of human lung cancer using 66989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66989-1-Ig

    IHC staining of human lung cancer using 66989-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66989-1-Ig (hIST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66989-1-Ig

    IF Staining of HeLa using 66989-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using hIST1 antibody (66989-1-Ig, Clone: 1E12A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, mouse liver tissue, HEK-293 cells, MCF-7 cells, Jurkat cells, A549 cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66989-1-Ig targets hIST1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen hIST1 fusion protein Ag28364 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name KIAA0174
Synonyms hIST1, IST1, IST1 homolog, KIAA0174, OLC1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC000430
Gene Symbol KIAA0174
Gene ID (NCBI) 9798
RRIDAB_2882306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

实验方案

Product Specific Protocols
WB protocol for hIST1 antibody 66989-1-IgDownload protocol
IHC protocol for hIST1 antibody 66989-1-IgDownload protocol
IF protocol for hIST1 antibody 66989-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

J Cell Biol

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters

Authors - Femmy C Stempels
View Article