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验证数据展示

  • WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

    WB analysis of human artery using 66382-1-Ig (same clone as 66382-1-PBS)

    Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGN antibody included
  • IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    IHC staining of human liver using 66382-1-Ig (same clone as 66382-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66382-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGN antibody included
  • Sandwich ELISA standard curve of MP50723-1

    Sandwich ELISA standard curve of MP50723-1

    Sandwich ELISA standard curve of MP50723-1, Human Osteoglycin Monoclonal Matched Antibody Pair - PBS only. 66382-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3484. 66382-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP50723-1

    Sample test of MP50723-1

    Plasma of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.7 ng/mL with a range of 1.2-2.2 ng/mL.

  • Sample test of MP50723-1

    Sample test of MP50723-1

    Serum of six individual healthy human donors was measured. The Osteoglycin concentration of detected samples was determined to be 1.9 ng/mL with a range of 1.4-2.3 ng/mL.

  • Cytometric bead array standard curve of MP50723-1

    Cytometric bead array standard curve of MP50723-1

    Cytometric bead array standard curve of MP50723-1, OGN/Osteoglycin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66382-1-PBS. Detection antibody: 66382-2-PBS. Standard:Ag3484. Range: 0.098-100 ng/mL.

Osteoglycin Monoclonal antibody, PBS Only (Capture)

Osteoglycin Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66382-1-PBS

产品说明书

CloneNo. 2A5F11

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

OGN, Osteoinductive factor, OIF, OG, Mimecan

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy OGN Ready-To-Use IHC Kit. OGN primary antibody included.

产品信息

66382-1-PBS targets Osteoglycin as part of a matched antibody pair:

MP50723-1: 66382-1-PBS capture and 66382-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, pig
免疫原 Osteoglycin fusion protein Ag3484 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 osteoglycin
别名 OGN, Osteoinductive factor, OIF, OG, Mimecan
计算分子量 298 aa, 34 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC037273
基因名称 OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20774
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.