• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OGN Monoclonal antibody

OGN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A5F11

Cat No : 66382-1-Ig

Print datasheet

Synonyms

Mimecan, OG, OGN, OIF, osteoglycin, Osteoinductive factor, SLRR3A

验证数据展示

  • WB analysis of human artery using 66382-1-Ig

    WB analysis of human artery using 66382-1-Ig

    Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (OGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66382-1-Ig

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with OGN antibody included
  • IHC staining of human liver using 66382-1-Ig

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (OGN Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with OGN antibody included

经过测试的应用

Positive WB detected inhuman artery tissue, pig artery tissue
Positive IHC detected inhuman liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OGN Ready-To-Use IHC Kit. OGN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66382-1-Ig targets OGN in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, pig
Immunogen OGN fusion protein Ag3484 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name osteoglycin
Synonyms Mimecan, OG, OGN, OIF, osteoglycin, Osteoinductive factor, SLRR3A
Calculated Molecular Weight 298 aa, 34 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC037273
Gene Symbol OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20774
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

实验方案

Product Specific Protocols
WB protocol for OGN antibody 66382-1-IgDownload protocol
IHC protocol for OGN antibody 66382-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols