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验证数据展示

  • WB analysis of human artery using 66382-1-Ig

    WB analysis of human artery using 66382-1-Ig

    Human artery tissue were subjected to SDS PAGE followed by western blot with 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66382-1-Ig

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with OGN antibody included
  • IHC staining of human liver using 66382-1-Ig

    IHC staining of human liver using 66382-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66382-1-Ig (Osteoglycin Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with OGN antibody included

Osteoglycin Monoclonal antibody

Osteoglycin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66382-1-Ig

产品说明书

CloneNo. 2A5F11

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

OGN, Osteoinductive factor, OIF, OG, Mimecan

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman artery tissue, pig artery tissue
Positive IHC detected inhuman liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy OGN Ready-To-Use IHC Kit. OGN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66382-1-Ig targets Osteoglycin in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 Osteoglycin fusion protein Ag3484 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 osteoglycin
别名 OGN, Osteoinductive factor, OIF, OG, Mimecan
计算分子量 298 aa, 34 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC037273
基因名称 OGN
Gene ID (NCBI) 4969
RRIDAB_2881758
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20774
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

实验方案

Product Specific Protocols
WB protocol for Osteoglycin antibody 66382-1-IgDownload protocol
IHC protocol for Osteoglycin antibody 66382-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols