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验证数据展示

  • WB analysis of Jurkat using 66323-2-Ig (same clone as 66323-2-PBS)

    WB analysis of Jurkat using 66323-2-Ig (same clone as 66323-2-PBS)

    WB result of Glutamine synthetase antibody (66323-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IF Staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    IF Staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66323-2-Ig (same clone as 66323-2-PBS)

    FC experiment of HepG2 using 66323-2-Ig (same clone as 66323-2-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Glutamine Synthetase (66323-2-Ig, Clone:1B6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66323-2-Ig (same clone as 66323-2-PBS)

    IHC staining of human liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GLUL antibody included
  • IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    IF Staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1, Glutamine synthetase Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66323-2-PBS. Detection antibody: 66323-1-PBS. Standard:Ag6309. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO验证

Glutamine Synthetase Monoclonal antibody, PBS Only (Capture)

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66323-2-PBS

产品说明书

CloneNo. 1B6G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), Cytometric bead array, Indirect ELISA

别名

GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

产品信息

66323-2-PBS targets Glutamine Synthetase as part of a matched antibody pair:

MP50483-1: 66323-2-PBS capture and 66323-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6309

Product name: Recombinant human Glutamine synthetase protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC011700

Sequence: MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate-ammonia ligase (glutamine synthetase)
别名 GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS
计算分子量 374 aa, 42 kDa
观测分子量42 kDa
GenBank蛋白编号BC011700
基因名称 Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881704
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP15104
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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