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验证数据展示

  • WB analysis of Jurkat using 66323-2-Ig

    WB analysis of Jurkat using 66323-2-Ig

    WB result of Glutamine synthetase antibody (66323-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

  • IHC staining of human liver cancer using 66323-2-Ig

    IHC staining of human liver cancer using 66323-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-2-Ig

    IHC staining of human liver cancer using 66323-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLUL antibody included
  • IF Staining of mouse liver using 66323-2-Ig

    IF Staining of mouse liver using 66323-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HepG2 using 66323-2-Ig

    FC experiment of HepG2 using 66323-2-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Glutamine Synthetase (66323-2-Ig, Clone:1B6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human liver using 66323-2-Ig

    IHC staining of human liver using 66323-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLUL antibody included
  • IF Staining of mouse brain using 66323-2-Ig

    IF Staining of mouse brain using 66323-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 66323-2-Ig

    IF Staining of mouse brain using 66323-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 66323-2-Ig

    IF Staining of mouse liver using 66323-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA
Cat No. 66323-2-Ig

产品说明书

CloneNo. 1B6G4
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA and More (1)

别名

GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue, mouse brain tissue
Positive IF-Fro detected inmouse liver tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

66323-2-Ig targets Glutamine Synthetase in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性rat, canine
免疫原

CatNo: Ag6309

Product name: Recombinant human Glutamine synthetase protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-373 aa of BC011700

Sequence: MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate-ammonia ligase (glutamine synthetase)
别名 GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS
计算分子量 374 aa, 42 kDa
观测分子量42 kDa
GenBank蛋白编号BC011700
基因名称 Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881704
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP15104
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
WB protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineIF

Neural Regen Res

Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma

Authors - Yu Huan
View Article
ratIF

Neural Regen Res

Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

Authors - Yuxue Mu
View Article
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