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验证数据展示

  • WB analysis of human kidney using 66084-1-Ig

    WB analysis of human kidney using 66084-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66084-1-Ig

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 66084-1-Ig

    IHC staining of human liver using 66084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 66084-1-Ig

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66084-1-Ig

    IF Staining of human liver cancer using 66084-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Serum amyloid P component Monoclonal antibody

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66084-1-Ig

产品说明书

CloneNo. 1A1D10
引用文献(2)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, human plasma
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, human liver tissue, mouse spleen tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

66084-1-Ig targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag14957

Product name: Recombinant human APCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-223 aa of BC007058

Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 amyloid P component, serum
别名 APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein
计算分子量 223 aa, 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC007058
基因名称 Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_11182271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02743
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum amyloid P component (SAP; also termed P-SAP or APCS), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

实验方案

Product Specific Protocols
IF protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
IHC protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
WB protocol for Serum amyloid P component antibody 66084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell

Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures

Authors - Yingjie Ding
View Article
humanIHC

Clin Chim Acta

Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC

Authors - Weifeng Wang
View Article
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