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验证数据展示

  • WB analysis of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

    WB analysis of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

Serum amyloid P component Monoclonal antibody, PBS Only

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66084-1-PBS

产品说明书

CloneNo. 1A1D10

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66084-1-PBS targets Serum amyloid P component in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14957

Product name: Recombinant human APCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-223 aa of BC007058

Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 amyloid P component, serum
别名 APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein
计算分子量 223 aa, 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC007058
基因名称 Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_11182271
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02743
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

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