验证数据展示
经过测试的应用
| Positive IHC-Autostainer detected in | human lung cancer tissue, human lung tissue, human appendicitis tissue, human kidney tissue |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue, human kidney tissue, mouse kidney tissue, human appendicitis tissue, human lung tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:10000-1:60000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 2 publications below |
产品信息
60259-2-Ig targets Napsin A in IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IHC-Autostainer, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9721 Product name: Recombinant human NAPSA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-420 aa of BC017842 Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | napsin A aspartic peptidase |
| 别名 | NAPSA, 1H7F2, Asp 4, ASP4, Aspartyl protease 4 |
| 计算分子量 | 420 aa, 45 kDa |
| GenBank蛋白编号 | BC017842 |
| 基因名称 | Napsin A |
| Gene ID (NCBI) | 9476 |
| RRID | AB_2881381 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O96009 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Napsin A antibody 60259-2-Ig | Download protocol |
| IF protocol for Napsin A antibody 60259-2-Ig | Download protocol |
| IHC protocol for Napsin A antibody 60259-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Patient-derived ovarian cancer organoids as platforms for predicting platinum resistance and screening tumor stem cell inhibitors. | ||
Nat Commun Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. | ||
Acta Biomater Biomechanical profiles and molecular regulation of clinical cancer stem cells and tumor cells: Implications for tumorigenicity and metastasis of lung cancer. | ||
Biomater Res Engineered Endometrial Clear Cell Cancer-on-a-Chip Reveals Early Invasion-Metastasis Cascade of Cancer Cells | ||
Br J Cancer Novel transcription factor zinc finger 514 suppresses lung adenocarcinoma progression and enhances cisplatin sensitivity via transcriptional repression of COL1A1. | ||
Respir Res CALU promotes lung adenocarcinoma progression by enhancing cell proliferation, migration and invasion |
