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验证数据展示

  • WB analysis of K-562 using 60176-1-Ig

    WB analysis of K-562 using 60176-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included

ACPP Monoclonal antibody

ACPP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60176-1-Ig

产品说明书

CloneNo. 1G2C9
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, human testis tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag8578

Product name: Recombinant human ACPP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-373 aa of BC007460

Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acid phosphatase, prostate
别名 PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3
计算分子量 418 aa, 48 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC007460
基因名称 ACPP
Gene ID (NCBI) 55
RRIDAB_10733336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15309
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
IF protocol for ACPP antibody 60176-1-IgDownload protocol
IHC protocol for ACPP antibody 60176-1-IgDownload protocol
WB protocol for ACPP antibody 60176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids

Authors - Mingyang Li
View Article
humanWB

Front Endocrinol (Lausanne)

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Authors - Chong Wang
View Article
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