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验证数据展示

  • WB analysis using 29321-1-AP

    WB analysis using 29321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29321-1-AP (SPATA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 29321-1-AP

    IHC staining of mouse lung using 29321-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 29321-1-AP

    IHC staining of mouse lung using 29321-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 29321-1-AP

    IF Staining of HeLa using 29321-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPATA2 antibody (29321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SPATA2 Polyclonal antibody

SPATA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29321-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

FLJ13167, KIAA0757, PD1, SPATA2, spermatogenesis associated 2, tamo

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29321-1-AP targets SPATA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag30773

Product name: Recombinant human SPATA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 330-520 aa of BC009481

Sequence: STQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 2
别名 FLJ13167, KIAA0757, PD1, SPATA2, spermatogenesis associated 2, tamo
计算分子量 58 kDa
观测分子量58-60 kDa
GenBank蛋白编号BC009481
基因名称 SPATA2
Gene ID (NCBI) 9825
RRIDAB_2918282
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)

实验方案

Product Specific Protocols
IF protocol for SPATA2 antibody 29321-1-APDownload protocol
IHC protocol for SPATA2 antibody 29321-1-APDownload protocol
WB protocol for SPATA2 antibody 29321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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