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验证数据展示

  • WB analysis using 29321-1-AP (same clone as 29321-1-PBS)

    WB analysis using 29321-1-AP (same clone as 29321-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29321-1-AP (SPATA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    IHC staining of mouse lung using 29321-1-AP (same clone as 29321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29321-1-AP (same clone as 29321-1-PBS)

    IF Staining of HeLa using 29321-1-AP (same clone as 29321-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPATA2 antibody (29321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29321-1-PBS in a different storage buffer formulation.

SPATA2 Polyclonal antibody, PBS Only

SPATA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29321-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA0757, PD1, spermatogenesis associated 2, Spermatogenesis-associated protein 2, tamo

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29321-1-PBS targets SPATA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30773

Product name: Recombinant human SPATA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 330-520 aa of BC009481

Sequence: STQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 2
别名 KIAA0757, PD1, spermatogenesis associated 2, Spermatogenesis-associated protein 2, tamo
计算分子量 58 kDa
观测分子量58-60 kDa
GenBank蛋白编号BC009481
基因名称 SPATA2
Gene ID (NCBI) 9825
RRIDAB_2918282
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM82
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)

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