Phospho-RIPK1 (Ser161) Monoclonal antibody

Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B2G1

Cat No : 66854-1-Ig

Print datasheet

Synonyms

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1



经过测试的应用

Positive WB detected inJurkat cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells
Positive IHC detected inhuman small intestine tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
UniprotQ13546
Gene ID (NCBI) 8737
RRIDAB_2882194
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

实验方案

Product Specific Protocols
WB protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
IHC protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
IF protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.

Authors - Chia-Kang Ho
humanWB

Front Genet

Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation.

Authors - Yongchang Yang
mouseWB,IHC

Am J Physiol Renal Physiol

Myo-inositol oxygenase overexpression exacerbates cadmium-induced kidney injury via oxidant stress and necroptosis.

Authors - Xiaoping Zheng