Phospho-RIPK1 (Ser161) Monoclonal antibody
Phospho-RIPK1 (Ser161) Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B2G1
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, Calyculin A treated HEK-293T cells, Raji cells, THP-1 cells |
Positive IHC detected in | human small intestine tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U2OS cells |
Positive FC detected in | Calyculin A treated HEK-293T cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 2000-20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
WB | See 8 publications below |
IHC | See 1 publications below |
产品信息
66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IHC, WB |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
Synonyms | Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1 |
Calculated molecular weight | 76 kDa |
Observed molecular weight | 74 kDa, 45 kDa |
GenBank accession number | NM_003804 |
Gene symbol | RIPK1 |
Uniprot | Q13546 |
Gene ID (NCBI) | 8737 |
RRID | AB_2882194 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
IHC protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
IF protocol for Phospho-RIPK1 (Ser161) antibody 66854-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Inflammation Ursolic Acid Promotes Autophagy by Inhibiting Akt/mTOR and TNF-α/TNFR1 Signaling Pathways to Alleviate Pyroptosis and Necroptosis in Mycobacterium tuberculosis-Infected Macrophages | ||
J Cell Mol Med LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis. | ||
Front Genet Exosome-Derived LncRNA TCONS_00072128 Mediated Osteogenic Differentiation and Inflammation by Caspase 8 Regulation. | ||
Am J Physiol Renal Physiol Myo-inositol oxygenase overexpression exacerbates cadmium-induced kidney injury via oxidant stress and necroptosis. | ||
Eur J Pharmacol A natural chalcone cardamonin inhibits necroptosis and ameliorates dextran sulfate sodium (DSS)-induced colitis by targeting RIPK1/3 kinases | ||
Front Oncol Identification of necroptosis-related genes for predicting prognosis and exploring immune infiltration landscape in colon adenocarcinoma |