CoraLite® Plus 488-conjugated Phospho-RIPK1 (Ser161) Monoclonal antibody

Phospho-RIPK1 (Ser161) Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2G1

Cat No : CL488-66854

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Synonyms

Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161) , Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1



经过测试的应用

Positive IF detected inU2OS cells
Positive FC detected inCalyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 66854-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

CL488-66854 targets Phospho-RIPK1 (Ser161) in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser161) , Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1
Calculated molecular weight 76 kDa
Observed molecular weight 74 kDa, 45 kDa
GenBank accession numberNM_003804
Gene symbol RIPK1
UniprotQ13546
Gene ID (NCBI) 8737
RRIDAB_2919380
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Phospho-RIPK1 (Ser161) antibody CL488-66854Download protocol
Standard Protocols
Click here to view our Standard Protocols