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CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

MOPC-21

Cat No : CL488-65124

Print datasheet

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

验证数据展示

  • FC experiment of human PBMCs using CL488-65124

    FC experiment of human PBMCs using CL488-65124

    1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-Mouse IgG1 Isotype Control (CL488-65124' target='_blank'>CL488-65124, Clone: MOPC-21) (red), CL488 Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 65124-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Synonyms IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control
Gene Symbol Ighg1
Gene ID (NCBI) 16017
RRIDAB_2919265
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01868
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 IgG1 Isotype Control antibody CL488-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols