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验证数据展示

  • FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

    FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

    1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98480-1-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

    FC experiment of Transfected HEK-293T using 98480-1-RR (same clone as 98480-1-PBS)

    1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98480-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98480-1-RR

    Affinity and Kinetic Characterization of 98480-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98480-1-RR against Mouse CCL8/MCP-2 were performed. The affinity constant is below 1 pM.

Anti-Mouse CCL8/MCP-2 Rabbit Recombinant Antibody, PBS Only

CCL8/MCP-2 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98480-1-PBS

产品说明书

CloneNo. 250174H6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Ccl8, C-C motif chemokine 8, Ccl 8, CCL-8, chemokine (C C motif) ligand 8

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98480-1-PBS targets CCL8/MCP-2 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2771

Product name: Recombinant Mouse CCL8/MCP-2 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-97 aa of NM_021443.3

Sequence: GPDKAPVTCCFHVLKLKIPLRVLKSYERINNIQCPMEAVVFQTKQGMSLCVDPTQKWVSEYMEILDQKSQILQP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 8
别名 Ccl8, C-C motif chemokine 8, Ccl 8, CCL-8, chemokine (C C motif) ligand 8
计算分子量11 kDa
GenBank蛋白编号NM_021443.3
基因名称 Ccl8
Gene ID (NCBI) 20307
RRIDAB_3746110
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Z121
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cys-cys (C-C) motif chemokine ligand 8 (CCL8, also known as MCP2) is implicated in various inflammatory processes. In bone marrow transplantation, circulating CCL8 levels positively correlate with the severity of graft-versus-host disease.

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