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验证数据展示

  • FC experiment of Transfected HEK-293T using 98480-1-RR

    FC experiment of Transfected HEK-293T using 98480-1-RR

    1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98480-1-RR

    FC experiment of Transfected HEK-293T using 98480-1-RR

    1x10^6 HEK-293T cells (left) or CCL8/MCP-2-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL8/MCP-2 Rabbit RecAb (98480-1-RR, Clone: 250174H6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Mouse CCL8/MCP-2 Rabbit Recombinant Antibody

CCL8/MCP-2 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98480-1-RR

产品说明书

CloneNo. 250174H6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Ccl8, C-C motif chemokine 8, Ccl 8, CCL-8, chemokine (C C motif) ligand 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
PE-Cyanine7
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98480-1-RR targets CCL8/MCP-2 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 CCL8/MCP-2 fusion protein Eg2771 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 8
别名 Ccl8, C-C motif chemokine 8, Ccl 8, CCL-8, chemokine (C C motif) ligand 8
计算分子量11 kDa
GenBank蛋白编号NM_021443.3
基因名称 Ccl8
Gene ID (NCBI) 20307
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Z121
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The cys-cys (C-C) motif chemokine ligand 8 (CCL8, also known as MCP2) is implicated in various inflammatory processes. In bone marrow transplantation, circulating CCL8 levels positively correlate with the severity of graft-versus-host disease.

实验方案

Product Specific Protocols
FC protocol for CCL8/MCP-2 antibody 98480-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols