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验证数据展示

  • IF Staining of mouse heart using CL488-55135

    IF Staining of mouse heart using CL488-55135

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin-specific antibody (CL488-55135) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-55135

    IF Staining of mouse spleen using CL488-55135

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-55135) at dilution of 1:200, F4/80 antibody (28463-1-AP, Magenta).

CoraLite® Plus 488-conjugated smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for IF-P
Cat No. CL488-55135

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue, mouse spleen tissue

This antibody is recommended for use in IHC.

Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55135-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55135 targets smooth muscle actin specific in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, alpha 2, smooth muscle, aorta
别名 ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2
计算分子量 42 kDa
观测分子量 42 kDa
GenBank蛋白编号NM_001613
基因名称 Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2919221
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62736
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 smooth muscle actin specific antibody CL488-55135Download protocol
Standard Protocols
Click here to view our Standard Protocols