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验证数据展示

  • WB analysis using 28463-1-AP

    WB analysis using 28463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28463-1-AP (F4/80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 28463-1-AP

    IHC staining of mouse spleen using 28463-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IHC staining of mouse spleen using 28463-1-AP

    IHC staining of mouse spleen using 28463-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28463-1-AP (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mouse F4/80 antibody included
  • IF Staining of mouse spleen using 28463-1-AP

    IF Staining of mouse spleen using 28463-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse spleen using 28463-1-AP

    IF Staining of mouse spleen using 28463-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of RAW 264.7 using 28463-1-AP

    IF Staining of RAW 264.7 using 28463-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (28463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse spleen using 28463-1-AP

    IF Staining of mouse spleen using 28463-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody (28463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

F4/80 Polyclonal antibody

F4/80 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 28463-1-AP

产品说明书

引用文献(312)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

别名

Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, Emr1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inunboiled J774A.1 cells, unboiled RAW 264.7 cells
Positive IHC detected inmouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse liver tissue
Positive IF-Fro detected inmouse spleen tissue
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy Mouse F4/80 Ready-To-Use IHC Kit. Mouse F4/80 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28463-1-AP targets F4/80 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原

CatNo: Ag28252

Product name: Recombinant mouse Adgre1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 443-644 aa of NM_010130

Sequence: MELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTME

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EGF-like module containing, mucin-like, hormone receptor-like sequence 1
别名 Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, Emr1
计算分子量 102 kDa
观测分子量130 kDa, 160 kDa
GenBank蛋白编号NM_010130
基因名称 F4/80
Gene ID (NCBI) 13733
RRIDAB_2881149
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ61549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

实验方案

Product Specific Protocols
IF protocol for F4/80 antibody 28463-1-APDownload protocol
IHC protocol for F4/80 antibody 28463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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