Validation Data Gallery
Technical Specifications
| GeneID | 7042 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.018-0.18 ng/mL |
| Specific Activity | minimally 2.00 x 107 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P27090 |
| Molecular Mass | 13 kDa reduced, 23 kDa non-reduced, homodimer, non-glycosylated |
| Formulation | 50mM Acetate pH 3.0 + 20% EtOH, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 20% ethanol + 50 mM NaOAc + 75 mM HOAc. Gently swirl or tap vial to mix. |
Background
Recombinant Human Transforming Growth Factor Beta-2 (TGF beta-2) is a multifunctional cytokine that plays a critical role in cardiac development, cell proliferation, and differentiation. It is widely used in the expansion and maintenance of pluripotent human embryonic stem cells (hESCs), making it essential for stem cell research, regenerative medicine, and cell and gene therapy (CGT) workflows. HumanKine TGF beta-2 is expressed in HEK293 human cells, ensuring authentic human post-translational modifications (PTMs) that enhance protein folding, stability, and biological activity compared to non-mammalian expression systems. This results in improved performance in sensitive applications such as stem cell culture and translational research.TGF beta-2 functions by binding to type I and type II TGF beta receptors, activating downstream SMAD signaling pathways that regulate gene expression, maintain pluripotency, and control cell fate decisions.
Synonyms
BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2
Publications
| Species | Title |
|---|---|
MedComm (2020) Transforming Growth Factor Beta2 Promotes Migration and Inhibits the Proliferation of Gastric Cancer Cells by Regulating the pSmad2/3-NDRG1 Signaling Pathway | |
Sci Adv Integrin inhibition facilitates fibrocartilaginous transformation in connective tissue in osteoarthritis. | |
J Transl Med Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification. | |
Cancer Gene Ther Enhancer hijacking drives FAM20C expression to promote papillary thyroid cancer progression. | |
Int Immunopharmacol Involvement of USP7 in aggravating Kawasaki disease by promoting TGFβ2 signaling mediated endothelial-mesenchymal transition and coronary artery remodeling | |
Invest Ophthalmol Vis Sci Aldose Reductase Mediates Transforming Growth Factor β2 (TGF-β2)-Induced Migration and Epithelial-To-Mesenchymal Transition of Lens-Derived Epithelial Cells. |
