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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated peroxiredoxin 2 Monoclonal antibody

peroxiredoxin 2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H6C4

Cat No : CL488-60202

Print datasheet

Synonyms

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

验证数据展示

  • IF Staining of MCF-7 using CL488-60202

    IF Staining of MCF-7 using CL488-60202

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL488-60202 (peroxiredoxin 2 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 60202-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-60202 is peroxiredoxin 2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, mouse
Immunogen peroxiredoxin 2 fusion protein Ag0835 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name peroxiredoxin 2
Synonyms NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003022
Gene Symbol PRDX2
Gene ID (NCBI) 7001
RRIDAB_2883118
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32119
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 peroxiredoxin 2 antibody CL488-60202Download protocol
Standard Protocols
Click here to view our Standard Protocols