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CoraLite® Plus 488-conjugated p504S,AMACR Monoclonal antibody

p504S,AMACR Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9B5

Cat No : CL488-60240

Print datasheet

Synonyms

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, p504S,AMACR, RACE

验证数据展示

  • FC experiment of LNCaP using CL488-60240

    FC experiment of LNCaP using CL488-60240

    1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p504S,AMACR (CL488-60240, Clone:2H9B5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inLNCaP cells
For other applications, we recommend the unconjugated version of this antibody, 60240-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60240 targets p504S,AMACR in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen p504S,AMACR fusion protein Ag8720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-methylacyl-CoA racemase
Synonyms 2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, p504S,AMACR, RACE
Calculated Molecular Weight 382 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC009471
Gene Symbol AMACR
Gene ID (NCBI) 23600
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHK6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 p504S,AMACR antibody CL488-60240Download protocol
Standard Protocols
Click here to view our Standard Protocols