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验证数据展示

  • WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

    FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

p130Cas / BCAR1 Monoclonal antibody, PBS Only

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67215-1-PBS

产品说明书

CloneNo. 2D11C10

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67215-1-PBS targets p130Cas / BCAR1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10604

Product name: Recombinant human BCAR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC062556

Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1
计算分子量 870 aa, 93 kDa
观测分子量 116 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56945
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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