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验证数据展示

  • WB analysis using 67215-1-Ig

    WB analysis using 67215-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67215-1-Ig

    IHC staining of human breast cancer using 67215-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67215-1-Ig

    IF Staining of human breast cancer using 67215-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of A431 using 67215-1-Ig

    FC experiment of A431 using 67215-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

p130Cas / BCAR1 Monoclonal antibody

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 67215-1-Ig

产品说明书

CloneNo. 2D11C10
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

别名

BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, T-47D cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67215-1-Ig targets p130Cas / BCAR1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag10604

Product name: Recombinant human BCAR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC062556

Sequence: MNHLNVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPAGPGSGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPAKQTSTFSKQTPHHPFPNPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDPLLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPPPDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 breast cancer anti-estrogen resistance 1
别名 BCAR1, 2D11C10, Breast cancer anti-estrogen resistance protein 1, CAS, CAS1
计算分子量 870 aa, 93 kDa
观测分子量 116 kDa
GenBank蛋白编号BC062556
基因名称 BCAR1
Gene ID (NCBI) 9564
RRIDAB_2882507
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IF protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
IHC protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
WB protocol for p130Cas / BCAR1 antibody 67215-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia.

Authors - Tingting Hu
View Article
humanIF

Comput Math Methods Med

Protumor Effects of Histone H3-H4 Chaperone Antisilencing Feature 1B Gene on Lung Adenocarcinoma: In Silico and In Vitro Analyses.

Authors - Liyang Wu
View Article
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