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验证数据展示

  • IF Staining of mouse testis using CL488-68055

    IF Staining of mouse testis using CL488-68055

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 m6A antibody (CL488-68055, Clone: 1D5E10 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated m6A Monoclonal antibody

m6A Monoclonal Antibody for IF-P
Cat No. CL488-68055

产品说明书

CloneNo. 1D5E10

宿主/亚型

Mouse / IgG3

种属反应性

chemical compound, m6a

应用

IF-P

别名

6-N-Methyladenoside, N6-methyladenosine

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 68055-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68055 targets m6A in IF-P applications and shows reactivity with chemical compound, m6a samples.

经测试应用 IF-P Application Description
经测试反应性 chemical compound, m6a
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 m6A
别名 6-N-Methyladenoside, N6-methyladenosine
GenBank蛋白编号m6A
基因名称
Gene ID (NCBI)
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 m6A antibody CL488-68055Download protocol
Standard Protocols
Click here to view our Standard Protocols