- Featured Product
- KD/KO Validated
CoraLite®594-conjugated iNOS Polyclonal antibody
iNOS Polyclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells, HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 2 publications below |
产品信息
CL594-18985 targets iNOS in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra), IF/ICC |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nitric oxide synthase 2, inducible |
Synonyms | HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A |
Calculated Molecular Weight | 131 kDa |
Observed Molecular Weight | 110-130 kDa, 65-70 kDa |
GenBank Accession Number | NM_000625 |
Gene Symbol | NOS2 |
Gene ID (NCBI) | 4843 |
RRID | AB_2919849 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P35228 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL594 iNOS antibody CL594-18985 | Download protocol |
FC protocol for CL594 iNOS antibody CL594-18985 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Ethnopharmacol Elsholtzia bodinieri Vaniot ameliorated acute lung injury in mice by regulating pyroptosis, inflammation, oxidative stress and macrophage polarization | ||
Biomaterials Magnesium/gallic acid bioMOFs laden carbonized mushroom aerogel effectively heals biofilm-infected skin wounds |