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验证数据展示

  • WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

    WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

    Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

    WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

    LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

iNOS Recombinant antibody, PBS Only

iNOS Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 80517-1-PBS

产品说明书

CloneNo. 6O22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80517-1-PBS targets iNOS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nitric oxide synthase 2, inducible
别名 NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS
计算分子量 131 kDa
观测分子量110-130 kDa
GenBank蛋白编号NM_000625
基因名称 iNOS
Gene ID (NCBI) 4843
RRIDAB_2918898
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35228
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.