• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

    WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

    Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

    WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

    LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

iNOS Recombinant monoclonal antibody, PBS Only

iNOS Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 80517-1-PBS

产品说明书

CloneNo. 6O22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

80517-1-PBS targets iNOS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nitric oxide synthase 2, inducible
别名 NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS
计算分子量 131 kDa
观测分子量110-130 kDa
GenBank蛋白编号NM_000625
基因名称 iNOS
Gene ID (NCBI) 4843
RRIDAB_2918898
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35228
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.