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验证数据展示

  • WB analysis using 12640-1-AP (same clone as 12640-1-PBS)

    WB analysis using 12640-1-AP (same clone as 12640-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    IF Staining of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    FC experiment of HeLa using 12640-1-AP (same clone as 12640-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human golgin 97 (12640-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12640-1-PBS in a different storage buffer formulation.

golgin 97 Polyclonal antibody, PBS Only

golgin 97 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 12640-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12640-1-PBS targets golgin 97 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3335

Product name: Recombinant human GOLGA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-767 aa of BC032853

Sequence: QIVALERMRAADQTTAEQGMRQLEQENAALKECRNEYERSLQNHQFELKKLKEEWSQREIVSVAMAQALEEVRKQREEFQQQAANLTAIIDEKEQNLREKTEVLLQKEQEILQLERGHNSALLQIHQLQAELEALRTLKAEEAAVVAEQEDLLRLRGPLQAEALSVNESHVTSRAMQDPVFQLPTAGRTPNGEVGAMDLTQLQKEKQDLEQQLLEKNKTIKQMQQRMLELRKTLQKELKIRPDNELFEVREKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 1
别名
计算分子量 767 aa, 88 kDa
观测分子量 97 kDa
GenBank蛋白编号BC032853
基因名称 golgin 97
Gene ID (NCBI) 2800
RRIDAB_2115315
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92805
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

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