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验证数据展示

  • IF Staining of HepG2 using CL488-67002

    IF Staining of HepG2 using CL488-67002

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 fetuin-B antibody (CL488-67002, Clone: 2H7D11 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated fetuin-B Monoclonal antibody

fetuin-B Monoclonal Antibody for IF/ICC
Cat No. CL488-67002

产品说明书

CloneNo. 2H7D11

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

IF/ICC

别名

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® Fetuin-B Ready-To-Use IHC Kit. Fetuin-B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67002-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67002 targets fetuin-B in IF/ICC applications and shows reactivity with human, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag12463

Product name: Recombinant human fetuin-B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-382 aa of BC074734

Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fetuin B
别名 FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11
计算分子量 382 aa, 42 kDa
观测分子量 50 kDa
GenBank蛋白编号BC074734
基因名称 Fetuin B
Gene ID (NCBI) 26998
RRIDAB_2919403
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UGM5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 fetuin-B antibody CL488-67002Download protocol
Standard Protocols
Click here to view our Standard Protocols
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