Dynamitin (1-401 AA) Monoclonal antibody
Dynamitin (1-401 AA) Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse
Applications
IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2B9A5
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
The immunogen of 60257-1-Ig is Dynamitin (1-401 AA) Fusion Protein expressed in E. coli.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | IF |
| Tested Reactivity | human, mouse |
| Immunogen | Dynamitin (1-401 AA) fusion protein Ag1902 种属同源性预测 |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dynactin 2 (p50) |
| Synonyms | DCTN2, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin |
| Calculated Molecular Weight | 401 aa, 44 kDa |
| Observed Molecular Weight | 52+150 kDa |
| GenBank Accession Number | BC014083 |
| Gene Symbol | DCTN2 |
| Gene ID (NCBI) | 10540 |
| RRID | AB_2881378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13561 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| IHC protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Biol Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster. |