验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
60158-1-Ig targets luciferase in WB, ELISA applications and shows reactivity with samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag13000 Product name: Recombinant dNGLUC protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-185 aa of AY015993 Sequence: MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | luciferase |
| 别名 | dNGLUC, Luciferase |
| 计算分子量 | 20 kDa |
| GenBank蛋白编号 | AY015993 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_10665950 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The immunogen of this antibody the luciferase(AY015993) from the copepod marine organism Gaussia princeps. This antibody is a McAb to Luciferase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for luciferase antibody 60158-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Multiplex Assay for Live-Cell Monitoring of Cellular Fates of Amyloid-β Precursor Protein (APP). | ||
Mol Ther Protein expression/secretion boost by a novel unique 21-mer cis-regulatory motif (Exin21) via mRNA stabilization |
