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CoraLite®555-conjugated cyclin B1 Monoclonal antibody

cyclin B1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1F3G11

Cat No : CL555-67686

Print datasheet

Synonyms

CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1

验证数据展示

  • IF Staining of HeLa using CL555-67686

    IF Staining of HeLa using CL555-67686

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 cyclin B1 antibody (CL555-67686, Clone: 1F3G11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-67686

    FC experiment of HeLa using CL555-67686

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human cyclin B1 (CL555-67686, Clone:1F3G11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67686-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67686 targets cyclin B1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen cyclin B1 fusion protein Ag29444 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin B1
Synonyms CCNB, CCNB1, cyclin B1, G2/mitotic specific cyclin B1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC006510
Gene Symbol CCNB1
Gene ID (NCBI) 891
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14635
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

实验方案

Product Specific Protocols
IF protocol for CL555 cyclin B1 antibody CL555-67686Download protocol
FC protocol for CL555 cyclin B1 antibody CL555-67686Download protocol
Standard Protocols
Click here to view our Standard Protocols