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CoraLite® Plus 647-conjugated citrate synthase Monoclonal antibody

citrate synthase Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F9G6

Cat No : CL647-67784

Print datasheet

Synonyms

citrate synthase, CS

验证数据展示

  • IF Staining of MCF-7 using CL647-67784

    IF Staining of MCF-7 using CL647-67784

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 citrate synthase antibody (CL647-67784, Clone: 2F9G6 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CS Ready-To-Use IHC Kit. CS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67784-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67784 targets citrate synthase in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen citrate synthase fusion protein Ag9255 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name citrate synthase
Synonyms citrate synthase, CS
Calculated Molecular Weight 466 aa, 52 kDa
GenBank Accession NumberBC010106
Gene Symbol CS
Gene ID (NCBI) 1431
RRIDAB_2920310
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75390
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 citrate synthase antibody CL647-67784Download protocol
Standard Protocols
Click here to view our Standard Protocols