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验证数据展示

  • WB analysis using 84779-4-RR

    WB analysis using 84779-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 84779-4-RR

    IP experiment of Raji using 84779-4-RR

    IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug.

  • FC experiment of Raji using 84779-4-RR

    FC experiment of Raji using 84779-4-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84779-4-RR

    Affinity and Kinetic Characterization of 84779-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84779-4-RR against Human cIAP2 were performed. The affinity constant is 46.8 pM.

cIAP2 Recombinant antibody

cIAP2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), IP, ELISA
Cat No. 84779-4-RR

产品说明书

CloneNo. 242214E11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

BIRC3, 242214E11, Baculoviral IAP repeat-containing protein 3, BIRC 3, C IAP2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells
Positive IP detected inRaji cells
Positive FC (Intra) detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy cIAP2/BIRC3 Ready-To-Use IHC Kit. cIAP2/BIRC3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84779-4-RR targets cIAP2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 cIAP2 fusion protein Ag20500 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 baculoviral IAP repeat-containing 3
别名 BIRC3, 242214E11, Baculoviral IAP repeat-containing protein 3, BIRC 3, C IAP2
计算分子量 604 aa, 68 kDa
观测分子量68 kDa
GenBank蛋白编号BC027485
基因名称 cIAP2
Gene ID (NCBI) 330
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13489
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.

实验方案

Product Specific Protocols
WB protocol for cIAP2 antibody 84779-4-RRDownload protocol
IP protocol for cIAP2 antibody 84779-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols