• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    WB analysis using 67447-1-Ig (same clone as 67447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67447-1-Ig (c-MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    IHC staining of human colon cancer using 67447-1-Ig (same clone as 67447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67447-1-Ig (c-MYC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of K-562 using 67447-1-Ig (same clone as 67447-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human c-MYC (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of NCCIT using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 NCCIT cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

    FC experiment of HeLa using 67447-1-Ig (same clone as 67447-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Monoclonal antibody (67447-1-Ig, Clone:3D9C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67447-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

c-MYC Monoclonal antibody, PBS Only

c-MYC Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67447-1-PBS

产品说明书

CloneNo. 3D9C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

67447-1-PBS targets c-MYC in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17519

Product name: Recombinant human MYC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 16-454 aa of BC000141

Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 v-myc myelocytomatosis viral oncogene homolog (avian)
别名 MYC, 3D9C12, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39
计算分子量 49 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC000141
基因名称 MYC
Gene ID (NCBI) 4609
RRIDAB_2882681
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP01106
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYC contains a basic helix-loop-helix (bHLH) domain. This protein is a multifunctional nuclear phosphoprotein, which plays a role in cell cycle process, apoptosis and cell transformation. As a transcription factor, it regulates the transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds to DNA in a nonspecific way, but also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutation, overexpression, rearrangement and translocation of this gene are related to many hematopoietic tumors, leukemia and lymphoma (including Burkitt lymphoma). The antibody is a rabbit polyclonal antibody against human MYC recombinant protein. The 50kDa band recognized by antibody is the natural form of MYC, while other bands between 60 and 70 kDa are the phosphorylated form of MYC (PMID: 12189186). The latest research reveals a new key mechanism of MYC protein, which is independent of its classical transcriptional regulation function. It was found that under the stress of transcription, MYC was polymerized through its specific RNA binding domain (RBRIII), and exocrine was targeted at the nuclear R loop and double-stranded RNA. This process can effectively eliminate the immunogenic RNA-DNA hybrid from R-loop, thus preventing it from activating TLR3-TBK1 natural immune signaling pathway. This mechanism is very important for MYC to maintain tumor growth and achieve immune escape in vivo. (PMID: 41576951)

||
New chat

Able™

正在加载，请稍候...