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验证数据展示

  • WB analysis of HeLa using 30597-1-AP (same clone as 30597-1-PBS)

    WB analysis of HeLa using 30597-1-AP (same clone as 30597-1-PBS)

    WB result of ZYG11A antibody (30597-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZYG11A transfected HeLa cells. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 30597-1-AP (same clone as 30597-1-PBS)

    IF Staining of K-562 using 30597-1-AP (same clone as 30597-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ZYG11A antibody (30597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZYG11A Polyclonal antibody, PBS Only

ZYG11A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30597-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Protein zyg 11 homolog A, ZYG11

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30597-1-PBS targets ZYG11A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30166

Product name: Recombinant human ZYG11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 628-696 aa of NM_001004339

Sequence: TSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zyg-11 homolog A (C. elegans)
别名 Protein zyg 11 homolog A, ZYG11
计算分子量 86 kDa
观测分子量48 kDa
GenBank蛋白编号NM_001004339
基因名称 ZYG11A
Gene ID (NCBI) 440590
RRIDAB_3742356
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6WRX3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZYG11A is a member of the Zyg11 protein family that functions as a target recruitment subunit of an E3 ubiquitin ligase complex playing a central role in the degradation of a regulatory protein involved in cell cycle regulation. ZYG11A is over-expressed in NSCLC tumor tissues and correlates with more aggressive clinical characteristics. Also ZYG11A gene constitutes a novel target for IGF1 action in endometrial cancer cells (PMID: 26771237, 31320996). This antibody is specific to isoform 2 of ZYG11A with a predicted molecular mass of 48 kDa.

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