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验证数据展示

  • WB analysis of HeLa using 30597-1-AP

    WB analysis of HeLa using 30597-1-AP

    WB result of ZYG11A antibody (30597-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZYG11A transfected HeLa cells.

  • WB analysis using 30597-1-AP

    WB analysis using 30597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30597-1-AP

    WB analysis using 30597-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 30597-1-AP

    IF Staining of K-562 using 30597-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ZYG11A antibody (30597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

ZYG11A Polyclonal antibody

ZYG11A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30597-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Protein zyg 11 homolog A, ZYG11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells, HEK-293T cells, HeLa cells
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30597-1-AP targets ZYG11A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30166

Product name: Recombinant human ZYG11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 628-696 aa of NM_001004339

Sequence: TSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zyg-11 homolog A (C. elegans)
别名 Protein zyg 11 homolog A, ZYG11
计算分子量 86 kDa
观测分子量48 kDa
GenBank蛋白编号NM_001004339
基因名称 ZYG11A
Gene ID (NCBI) 440590
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6WRX3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZYG11A is a member of the Zyg11 protein family that functions as a target recruitment subunit of an E3 ubiquitin ligase complex playing a central role in the degradation of a regulatory protein involved in cell cycle regulation. ZYG11A is over-expressed in NSCLC tumor tissues and correlates with more aggressive clinical characteristics. Also ZYG11A gene constitutes a novel target for IGF1 action in endometrial cancer cells (PMID: 26771237, 31320996). This antibody is specific to isoform 2 of ZYG11A with a predicted molecular mass of 48 kDa.

实验方案

Product Specific Protocols
IF protocol for ZYG11A antibody 30597-1-APDownload protocol
WB protocol for ZYG11A antibody 30597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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