验证数据展示
经过测试的应用
| Positive WB detected in | human kidney tissue, A431 cells, HEK-293 cells, mouse kidney tissue |
| Positive IF/ICC detected in | Hela cells |
ZXDC has low expression in most cells and tissues
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20530-1-AP targets ZXDC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14392 Product name: Recombinant human ZXDC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 173-522 aa of BC002940 Sequence: MDLAALFSDTPANASGSAGGSDEALNSGILTIDVTSVSSSLGGNLPANNSSLGPMEPLVLVAHSDIPPSLDSPLVLGTAATVLQQGSFSVDDVQTVSAGALGCLVALPMKNLSDDPLALTSNSNLAAHITTPTSSSTPRENASVPELLAPIKVEPDSPSRPGAVGQQEGSHGLPQSTLPSPAEQHGAQDTELSAGTGNFYLESGGSARTDYRAIQLAKEKKQRGAGSNAGASQSTQRKIKEGKMSPPHFHASQNSWLCGSLVVPSGGRPGPAPAAGVQCGAQGVQVQLVQDDPSGEGVLPSARGPATFLPFLTVDLPVYVLQEVLPSSGGPAGPEATQFPGSTINLQDLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ZXD family zinc finger C |
| 别名 | Zinc finger protein ZXDC, ZXD family zinc finger C, ZXD like zinc finger protein, ZXDC, ZXDL |
| 计算分子量 | 858 aa, 90 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC002940 |
| 基因名称 | ZXDC |
| Gene ID (NCBI) | 79364 |
| RRID | AB_10694823 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q2QGD7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZXDC antibody 20530-1-AP | Download protocol |
| WB protocol for ZXDC antibody 20530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Cell-fate transition and determination analysis of mouse male germ cells throughout development. | ||
iScience ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signaling | ||
Cancer Cell The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia |
