验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag5454 Product name: Recombinant human ZWILCH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 179-477 aa of BC036900 Sequence: KSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Zwilch, kinetochore associated, homolog (Drosophila) |
| 别名 | Protein zwilch homolog, KNTC1AP, hZwilch |
| 计算分子量 | 67 kDa |
| 观测分子量 | ~60 kDa |
| GenBank蛋白编号 | BC036900 |
| 基因名称 | ZWILCH |
| Gene ID (NCBI) | 55055 |
| RRID | AB_2878040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H900 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZWILCH antibody 14281-1-AP | Download protocol |
| WB protocol for ZWILCH antibody 14281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomedicines The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients |
