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验证数据展示

  • IHC staining of human colon cancer using 14281-1-AP

    IHC staining of human colon cancer using 14281-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14281-1-AP (ZWILCH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of PC-3 using 14281-1-AP

    WB analysis of PC-3 using 14281-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14281-1-AP (ZWILCH Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ZWILCH Polyclonal antibody

ZWILCH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14281-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Protein zwilch homolog, KNTC1AP, hZwilch

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag5454

Product name: Recombinant human ZWILCH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 179-477 aa of BC036900

Sequence: KSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAEQLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQMLLEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFSLTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFHKPDFSELTLNGSLEERIFFTNMVTCSQVHFK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Zwilch, kinetochore associated, homolog (Drosophila)
别名 Protein zwilch homolog, KNTC1AP, hZwilch
计算分子量 67 kDa
观测分子量 ~60 kDa
GenBank蛋白编号BC036900
基因名称 ZWILCH
Gene ID (NCBI) 55055
RRIDAB_2878040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ZWILCH antibody 14281-1-APDownload protocol
IHC protocol for ZWILCH antibody 14281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanIHC

Biomedicines

The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Authors - Małgorzata Blatkiewicz
View Article