ZWILCH Polyclonal antibody

ZWILCH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14281-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

Protein zwilch homolog, KNTC1AP, hZwilch

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ZWILCH fusion protein Ag5454 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Zwilch, kinetochore associated, homolog (Drosophila)
别名 Protein zwilch homolog, KNTC1AP, hZwilch
计算分子量 67 kDa
观测分子量 ~60 kDa
GenBank蛋白编号BC036900
基因名称 ZWILCH
Gene ID (NCBI) 55055
RRIDAB_2878040
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.

实验方案

Product Specific Protocols
WB protocol for ZWILCH antibody 14281-1-APDownload protocol
IHC protocol for ZWILCH antibody 14281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
humanIHC

Biomedicines

The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Authors - Małgorzata Blatkiewicz
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