CoraLite® Plus 488-conjugated ZP3 Polyclonal antibody
ZP3 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | mouse ovary tissue |
For other applications, we recommend the unconjugated version of this antibody, 21279-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21279 targets ZP3 in IF applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P |
| Tested Reactivity | human, mouse |
| Immunogen | ZP3 fusion protein Ag15791 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | zona pellucida glycoprotein 3 (sperm receptor) |
| Synonyms | Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC |
| Calculated Molecular Weight | 373 aa, 41 kDa |
| Observed Molecular Weight | 47 kDa, 60 kDa |
| GenBank Accession Number | BC113949 |
| Gene Symbol | ZP3 |
| Gene ID (NCBI) | 7784 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21754 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that are processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ZP3 antibody CL488-21279 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |