ZO-1 Recombinant antibody
ZO-1 Recombinant Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2P13
验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF detected in | MCF-7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82870-1-RR targets ZO-1 in WB, IP, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, IP, WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | ZO-1 fusion protein Ag33182 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | tight junction protein 1 (zona occludens 1) |
| Synonyms | DKFZp686M05161, Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, Zona occludens protein 1, Zonula occludens protein 1 |
| Calculated Molecular Weight | 1748 aa, 195 kDa |
| Observed Molecular Weight | 230 kDa |
| GenBank Accession Number | BC111712 |
| Gene Symbol | TJP1 |
| Gene ID (NCBI) | 7082 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q07157 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZO-1 antibody 82870-1-RR | Download protocol |
| IF protocol for ZO-1 antibody 82870-1-RR | Download protocol |
| IP protocol for ZO-1 antibody 82870-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |