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验证数据展示

  • WB analysis using CL750-83111

    WB analysis using CL750-83111

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83111 (ZO-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using CL750-83111

    FC experiment of A431 using CL750-83111

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody (CL750-83111, Clone:230474D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO验证
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CoraLite® Plus 750-conjugated ZO-2 Recombinant monoclonal antibody

ZO-2 Uni-rAb® Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-83111

产品说明书

CloneNo. 230474D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® TJP2/ZO-2 Ready-To-Use IHC Kit. TJP2/ZO-2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83111-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13534

Product name: Recombinant human TJP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-289 aa of BC027592

Sequence: MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tight junction protein 2 (zona occludens 2)
别名 TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2
计算分子量 134 kDa
观测分子量 ~150 kDa
GenBank蛋白编号BC027592
基因名称 TJP2
Gene ID (NCBI) 9414
RRIDAB_3673769
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UDY2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
FC protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
Standard Protocols
Click here to view our Standard Protocols