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ZNF839 Polyclonal antibody

ZNF839 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20539-1-AP

Print datasheet

Synonyms

C14orf131, zinc finger protein 839, ZNF839

验证数据展示

  • WB analysis of HeLa using 20539-1-AP

    WB analysis of HeLa using 20539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20539-1-AP (ZNF839 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20539-1-AP

    IF Staining of HepG2 using 20539-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF839 antibody 20539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, human small intestine tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20539-1-AP is ZNF839 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen ZNF839 fusion protein Ag14573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 839
Synonyms C14orf131, zinc finger protein 839, ZNF839
Calculated Molecular Weight 927 aa, 99 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC006222
Gene Symbol ZNF839
Gene ID (NCBI) 55778
RRIDAB_10695759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8K0R7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF839 antibody 20539-1-APDownload protocol
IHC protocol for ZNF839 antibody 20539-1-APDownload protocol
IF protocol for ZNF839 antibody 20539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols