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ZNF460 Polyclonal antibody

ZNF460 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25299-1-AP

Print datasheet

Synonyms

HZF8, Zinc finger protein 272, zinc finger protein 460, Zinc finger protein HZF8, ZNF272, ZNF460

验证数据展示

  • WB analysis using 25299-1-AP

    WB analysis using 25299-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 25299-1-AP (ZNF460 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25299-1-AP

    IHC staining of mouse brain using 25299-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25299-1-AP (ZNF460 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25299-1-AP

    IF Staining of HeLa using 25299-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF460 antibody (25299-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 25299-1-AP

    WB analysis using 25299-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25299-1-AP (ZNF460 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25299-1-AP is ZNF460 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ZNF460 fusion protein Ag18106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 460
Synonyms HZF8, Zinc finger protein 272, zinc finger protein 460, Zinc finger protein HZF8, ZNF272, ZNF460
Calculated Molecular Weight 562 aa, 64 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC118625
Gene Symbol ZNF460
Gene ID (NCBI) 10794
RRIDAB_2880016
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14592
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF460 antibody 25299-1-APDownload protocol
IHC protocol for ZNF460 antibody 25299-1-APDownload protocol
IF protocol for ZNF460 antibody 25299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,human

Cell Rep

Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism

Authors - Hao Qin
View Article
humanWB,IHC

J Cancer

Overexpression of ZNF460 predicts worse survival and promotes metastasis through JAK2/STAT3 signaling pathway in patient with colon cancer.

Authors - Tengfei Hao
View Article