Various lysates were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68424-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68424-1-PBS in a different storage buffer formulation.