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验证数据展示

  • WB analysis using 86901-1-RR (same clone as 86901-1-PBS)

    WB analysis using 86901-1-RR (same clone as 86901-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86901-1-RR (ZMYND8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86901-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 86901-1-RR (same clone as 86901-1-PBS)

    IF Staining of A431 using 86901-1-RR (same clone as 86901-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZMYND8 antibody (86901-1-RR, Clone: 251489A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86901-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86901-1-RR

    Affinity and Kinetic Characterization of 86901-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86901-1-RR against Human ZMYND8 were performed. The affinity constant is 5.34 nM.

ZMYND8 Recombinant monoclonal antibody, PBS Only

ZMYND8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86901-1-PBS

产品说明书

CloneNo. 251489A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

CTCL associated antigen se14 3, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, KIAA1125, MYND-type zinc finger-containing chromatin reader ZMYND8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZMYND8 Ready-To-Use IHC Kit. ZMYND8 primary antibody included.

产品信息

86901-1-PBS targets ZMYND8 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2207

Product name: Recombinant human ZMYND8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 745-1094 aa of BC030721

Sequence: WNSSTVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger, MYND-type containing 8
别名 CTCL associated antigen se14 3, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, KIAA1125, MYND-type zinc finger-containing chromatin reader ZMYND8
计算分子量 1186 aa, 132 kDa
观测分子量 150-170 kDa
GenBank蛋白编号BC030721
基因名称 ZMYND8
Gene ID (NCBI) 23613
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULU4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins, including REST corepressors (RCOR). ZMYND8 binds to ROCR2 and can function as a transcriptional repressor. It is co-expressed with RCOR2 in the nervous system, and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

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